e coli strains atcc 43896  (ATCC)

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: O78 avian pathogenic E. coli strains characterized in this study.

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques: Isolation

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Comparative genomics of the representative O78 APEC strains.

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques: Plasmid Preparation

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Comparison of genomic coverages (%) and identities (%) of O78 E. coli strains. A heatmap was constructed to represent the average nucleotide identity (ANI) and alignment coverage between O78 APEC and other RST strains. The values are visually depicted based on color intensity, with the alignment coverage ranging from red to green and the nucleotide identity represented by a color gradient from red to blue.

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques: Comparison, Construct

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Evolutionary model based on the spacer sequences of CRISPRs in O78 APEC strains according to RST: ( A ) RST3-1, ( B ) RST4-4, and ( C ) RST21-1. The CRISPR spacers of genetically related strains of each RST were compared, and their progenitor–progeny relationships were hypothesized in terms of spacer contents. In the case of RST3-1 strains, E19057 and E123 may be different progenies of APEC O78 and may have lost different numbers of spacers in CRT2. In the case of RST4-4, E19025, E18005, and NCTC11129 may have lost different numbers of spacers in CRT1 and CRT2 of a hypothetical progenitor. A RST3-1_G2 strain, WS3294A, shares the same hypothetical progenitor of RST4-4, reflecting their evolutionary relatedness. Only RST4-4 strains E19025, E18005, and NCTC11129 acquired one spacer (CRT2_11). In the case of RST21-1, E14033 may be a progeny of hypothetical progenitor I, and APEC O2-211, PSU-O78, and E12049 are progenies of hypothetical progenitor II. All the RST21-1 strains may have experienced different deletions in CRT1 and CRT2.

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques: CRISPR

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Frequency of RSTs among MDR O78 APEC strains ( n = 18).

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques:

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Frequency of antibiotic resistance-related genetic markers of O78 APEC strains ( n = 32 including the E123 strain).

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques:

Journal: Antibiotics

Article Title: Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli

doi: 10.3390/antibiotics12121714

Figure Lengend Snippet: Theoretical interconnections between O78 APEC strains (n = 32) with different contents of antibiotic resistance genes (genotypes). This schematic illustrates relationships between different genotypes.

Article Snippet: In the RST3-1_G4 genome type, three O78 strains (ATCC 43896, L3_E36, and 00-3279) were identified, and some of them were pathogenic and showed high genomic coverage/identity (98–99%/99.99%) and similar mPPTs to each other ( ).

Techniques: